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344045

Sigma-Aldrich

FOCUS-Protease Arrest Cocktail

A protease cocktail specifically developed for 2D gel sample preparation.

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About This Item

UNSPSC Code:
12352200

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

storage temp.

2-8°C

General description

A protease cocktail specifically developed for 2D gel sample preparation.
A protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.

Biochem/physiol Actions

Primary Target
Serine, cysteine, metalloproteinases, and calpain proteases
Product does not compete with ATP.

Physical form

Supplied as 100X.

Preparation Note

Following dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


Certificates of Analysis (COA)

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