344045
FOCUS-Protease Arrest Cocktail
A protease cocktail specifically developed for 2D gel sample preparation.
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About This Item
UNSPSC Code:
12352200
Quality Level
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
storage temp.
2-8°C
General description
A protease cocktail specifically developed for 2D gel sample preparation.
A protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.
Biochem/physiol Actions
Primary Target
Serine, cysteine, metalloproteinases, and calpain proteases
Serine, cysteine, metalloproteinases, and calpain proteases
Product does not compete with ATP.
Physical form
Supplied as 100X.
Preparation Note
Following dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
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