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Merck

344145

FPTase, Rat, Recombinant, E. coli

A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras.

Synonym(s):

Cathepsin L2, Cathepsin U, Farnesyl Proteintransferase, FTase

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About This Item

NACRES:
NA.77
UNSPSC Code:
12352202

Product Name

FPTase, Rat, Recombinant, E. coli, A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras.

recombinant

expressed in E. coli

assay

≥85% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Quality Level

200

Biochem/physiol Actions

1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.

Disclaimer

Toxicity: Standard Handling (A)

General description

M.W. α-subunit: ~48,000; β-subunit: ~46,000
Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.

Other Notes

Hightower, K.E., et al. 2001. Biochem. J.360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.

Physical form

In 25 mM HEPES, pH 7.2, 40 mM NaCl, 5µM ZnCl2, and 1mM TECP

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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