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Merck

344146

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

FPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.

Synonym(s):

FTase, Farnesyl Proteintransferase

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352202

Product Name

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli, FPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Biochem/physiol Actions

1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.

Other Notes

Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.

Physical form

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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