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344205

Sigma-Aldrich

Formamide - CAS 75-12-7 - Calbiochem

Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques. Specific Gravity: 1.1363; freezing point: 3.5°C.

Synonym(s):

Formamide, CH₃NO, Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
75-12-7
Molecular Weight:
45.04
Beilstein:
505995
MDL number:
MFCD00007941
UNSPSC Code:
12352200

vapor density

1.55 (vs air)

Quality Level

100

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

form

liquid

autoignition temp.

932 °F

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

hybridization: suitable

color

clear

refractive index

n20/D 1.447 (lit.)

pH

8-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

shipped in

ambient

storage temp.

10-30°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

General description

Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques (specific gravity: 1.1363; freezing point: 3.5°C).
Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques.
Specific Gravity: 1.1363; freezing point: 3.5°C.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

Noppinger, K., et al. 1992. Biotechniques 13, 572.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Carcinogenic / Teratogenic (D)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H351,H360D,H373

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Certificates of Analysis (COA)

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