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About This Item
CAS Number:
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
Specific activity:
≥1.0 units/mL, ≥1.5 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
form
liquid
specific activity
≥1.0 units/mL, ≥1.5 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Note: 1 mU = 1 milliunit.
Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
Other Notes
One unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
Zheng, Q., et al. 2002. J. Biol. Chem.277, 39823.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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