344826
α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli
This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides.
Synonym(s):
α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli, α-L-Fucoside Fucohydrolase
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About This Item
CAS Number:
EC Number:
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
eCl@ss:
32039090
Specific activity:
≥1.0 units/mL, ≥1.5 units/mg protein
Recombinant:
expressed in E. coli
Concentration:
≥1.0 U/mL
recombinant
expressed in E. coli
Quality Segment
form
liquid
specific activity
≥1.0 units/mL, ≥1.5 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
concentration
≥1.0 U/mL
optimum pH
5
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
General description
Substrate Specificity
an α-L-fucoside + H2O = L-fucose + an alcohol
an α-L-fucoside + H2O = L-fucose + an alcohol
Note: 1 mU = 1 milliunit.
Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
Zheng, Q., et al. 2002. J. Biol. Chem.277, 39823.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
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