345763
GDF-9, Mouse, Recombinant
GDF-9, Mouse, Recombinant, consists of amino acids 307-477 and is expressed in Chinese Hamster Ovary cells. An oocyte secreted paracrine factor that is necessary for mammalian folliculogenesis.
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About This Item
UNSPSC Code:
12352203
recombinant
expressed in CHO cells
Quality Level
Assay
≥95% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, mouse growth differentiation factor 9 (GDF-9) consisting of amino acids 307-477 and expressed in Chinese Hamster Ovary cells. GDF-9 is an oocyte secreted paracrine factor, necessary for mammalian ovarian folliculogenesis. Mature mouse GDF-9 is 90% identical to the amino acid sequence of mature human GDF-9. GDF-9 as a paracrine factor is useful in granulosa cell proliferation and differentiation regulation.
Biochem/physiol Actions
Effective concentration = 100 ng/ml as measured by induction of Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.
Physical form
Lyophilized from 0.2 µm sterile-filtered HCl, BSA.
Preparation Note
Reconstitute to 100 µg/ml in 4 mM HCl containing 0.1% bovine serum albumin.
Other Notes
Mottershead, D.G., et al. 2008. Mol. Cell. Endocrinol.283, 58.
Gilchrist, R.B., et al. 2006. J. Cell. Sci.119, 3811.
Mazerbourg, S., et al. 2004. Mol. Endocrinol.18, 653.
Kaivo-Oja, N., et al. 2003. J. Clin. Endocrinol. Metab.88, 755.
Roh, J.S., et al. 2003. Endocrinology144, 172.
Vitt, U.A., et al. 2002. Biol. Reprod.67, 473.
Dong, J., et al. 1996. Nature383, 531.
McGrath, S.A., et al. 1995. Mol. Endocrinol.9, 131.
McPherron, A.C. and S.J. Lee. 1992. J. Biol. Chem.268, 3444.
Gilchrist, R.B., et al. 2006. J. Cell. Sci.119, 3811.
Mazerbourg, S., et al. 2004. Mol. Endocrinol.18, 653.
Kaivo-Oja, N., et al. 2003. J. Clin. Endocrinol. Metab.88, 755.
Roh, J.S., et al. 2003. Endocrinology144, 172.
Vitt, U.A., et al. 2002. Biol. Reprod.67, 473.
Dong, J., et al. 1996. Nature383, 531.
McGrath, S.A., et al. 1995. Mol. Endocrinol.9, 131.
McPherron, A.C. and S.J. Lee. 1992. J. Biol. Chem.268, 3444.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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