β1,3-Galactosidase, Xanthomonas manihotis, Recombinant, E. coli

Name: β1,3-Galactosidase, <i>Xanthomonas manihotis</i>, Recombinant, <i>E. coli</i>
Brand: MM

Synonym(s):

β1,3-Galactosidase, Xanthomonas manihotis, Recombinant, E. coli, β-D-Galactoside Galactohydrolase

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About This Item

CAS Number:

9031-11-2

UNSPSC Code:

12352202

EC Number:

3.2.1.23 (BRENDA, IUBMB)

recombinant

expressed in E. coli

form

liquid

specific activity

≥2000 units/mL

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

β-N-acetylglucosaminidase, α-fucosidase, α-galactosidase, β-glucosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, Xanthomonas manihotis β1,3-Galactosidase expressed in E. coli. Cleaves terminal β1,3-linked galactose residues on oligosaccharides. Has >100-fold kinetic preference for β1,3- over β1,6-linkages and 500-fold kinetic preference for β1,3- over β1,4-linkages.

Other Notes

One unit is defined as the amount of enzyme that will cleave >95% of the β-D-galactose from 1 nmol Galβ1,3GlcNAcβ1,3Galβ1,4Glc-7-amino-4-methylcoumarin in 1 h at 37°C, pH 4.5 in a total volume of 10 µl.

Taron, C., et al. 1995. Glycobiology 5, 603.
Wong-Madden, S.T., and Landry, D. 1995. Glycobiology 5, 19.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 100 µM EDTA, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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