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Merck

362300

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli

Synonym(s):

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli, Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F

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About This Item

CAS Number:
83534-39-8
EC Number:
3.5.1.52 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00131222

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form

liquid

recombinant

expressed in E. coli

specific activity

≥10 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

β-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Related Categories

Disclaimer

Toxicity: Standard Handling (A)

General description

Note: 1 mU = 1 milliunit.
Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers.

Other Notes

Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.

Physical form

Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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