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362314

Sigma-Aldrich

Glycosyltransferase Affinity Gel-UDP

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About This Item

UNSPSC Code:
41116133

form

suspension

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

color

gray-white

shipped in

wet ice

storage temp.

2-8°C

General description

Recommended conditions for purification of galactosyltransferases: binding: 25 mM MnCl2, 25 mM MOPS, 0.1 mM DTT, pH 7.4; elution: 25 mM EDTA, 150 mM Tris-HCl, 0.1 mM DTT, 5 mM N-acetylglucosamine, pH 8.4.
Suitable for the affinity purification of galactosyltransferases. UDP coupled with a six-carbon spacer through a pyrophosphate linkage at 10 mmol UDP/ml gel slurry. Recommended conditions for purification of galactosyltransferases: binding: 25 mM MnCl2, 25 mM MOPS, 0.1 mM DTT, pH 7.4; elution: 25 mM EDTA, 150 mM Tris-HCl, 5 mM N-acetylglucosamine, 0.1 mM DTT, pH 8.4.
UDP coupled with a six-carbon spacer through a pyrophosphate linkage at 10 µmol UDP/ml gel slurry. Suitable for the affinity purification of galactosyltransferases.

Other Notes

Sadler, J.E., et al. 1982. Methods Enzymol. 83, 458.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

95.0 °F - Information taken from reference works and the literature.

Flash Point(C)

35 °C - Information taken from reference works and the literature.


Certificates of Analysis (COA)

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