362314
Glycosyltransferase Affinity Gel-UDP
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About This Item
UNSPSC Code:
41116133
form
suspension
Quality Segment
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
color
gray-white
shipped in
wet ice
storage temp.
2-8°C
General description
Recommended conditions for purification of galactosyltransferases: binding: 25 mM MnCl2, 25 mM MOPS, 0.1 mM DTT, pH 7.4; elution: 25 mM EDTA, 150 mM Tris-HCl, 0.1 mM DTT, 5 mM N-acetylglucosamine, pH 8.4.
Suitable for the affinity purification of galactosyltransferases. UDP coupled with a six-carbon spacer through a pyrophosphate linkage at 10 mmol UDP/ml gel slurry. Recommended conditions for purification of galactosyltransferases: binding: 25 mM MnCl2, 25 mM MOPS, 0.1 mM DTT, pH 7.4; elution: 25 mM EDTA, 150 mM Tris-HCl, 5 mM N-acetylglucosamine, 0.1 mM DTT, pH 8.4.
UDP coupled with a six-carbon spacer through a pyrophosphate linkage at 10 µmol UDP/ml gel slurry. Suitable for the affinity purification of galactosyltransferases.
Other Notes
Sadler, J.E., et al. 1982. Methods Enzymol. 83, 458.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
95.0 °F - Information taken from reference works and the literature.
flash_point_c
35 °C - Information taken from reference works and the literature.
Certificates of Analysis (COA)
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