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Merck

371207

Dkk-1, Mouse, Recombinant

Dkk-1, Mouse, Recombinant, consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

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Product Name

Dkk-1, Mouse, Recombinant, Dkk-1, Mouse, Recombinant, consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

recombinant

expressed in CHO cells

assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biochem/physiol Actions

ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml.

Other Notes

Pinzone, J.J., et al. 2009. Blood113, 517.
Li, J., et al. 2006. Bone36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C., 2006. Oncogene25, 7469.
Verani, R., et al. 2006. J. Neurochem.101, 242.
Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.
Kemp, C., et al. 2005. Dev. Dyn.233, 1064.
Adamska, M., et al. 2004. Dev. Biol.272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.
Mao, B., et al. 2002. Nature417, 664.
Mao, B., et al. 2001. Nature411, 321.
Glinka, A., et al. 1998. Nature391, 357.

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

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