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Merck

371208

Dkk-1, Rat, Recombinant

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

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About This Item

NACRES:
NA.27
UNSPSC Code:
12352203

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Product Name

Dkk-1, Rat, Recombinant, Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

recombinant

expressed in mouse NSO cells

assay

≥95% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

ND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.

Disclaimer

Toxicity: Standard Handling (A)

General description

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.

Other Notes

Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Krupnik, V.E., et al. 1999. Gene238, 301.

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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