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Merck

373275

Hh Signaling Antagonist XII, HPI-1

The Hh Signaling Antagonist XII, HPI-1, also referenced under CAS 599150-20-6, controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.

Synonym(s):

Hh Signaling Antagonist XII, HPI-1, 2-Methoxyethyl-4-(3-hydroxyphenyl)-7-(2-methoxyphenyl)-2-methyl-5-oxo-4,6,7,8-tetrahydro-1H-quinoline-3-carboxylate

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About This Item

Empirical Formula (Hill Notation):
C27H29NO6
CAS Number:
599150-20-6
Molecular Weight:
463.52
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
solid
Quality level:
100
Storage condition:
OK to freeze
protect from light

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Product Name

Hh Signaling Antagonist XII, HPI-1, The Hh Signaling Antagonist XII, HPI-1, also referenced under CAS 599150-20-6, controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.

InChI key

ZXSWZQSYZYMZKS-UHFFFAOYSA-N

InChI

1S/C27H29NO6/c1-16-24(27(31)34-12-11-32-2)25(17-7-6-8-19(29)13-17)26-21(28-16)14-18(15-22(26)30)20-9-4-5-10-23(20)33-3/h4-10,13,18,25,28-29H,11-12,14-15H2,1-3H3

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

pale yellow

solubility

DMSO: 100 mg/mL

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Disclaimer

Toxicity: Standard Handling (A)

General description

A cell-permeable oxo-tetrahydroquinolinecarboxylate compound that acts as a downstream Hh (Hedgehog) pathway blocker, effectively inhibiting Gli-dependent transcription activity in both Sufu+/+ NIH 3T3 (shh-LIGHT2) and Sufu-/- fibroblast cultures (IC50 = 1.5 & 3 µM, respectively) in a manner similar to two other Hh downstream blockers, HPI-3 (Cat. No. 373276) and GANT-61 (Cat. No. 373401). However, only HPI-1, but not HPI-3 or GANT-61 (no effect at 30 µM), remains effective against Sufu-/- fibroblasts overexpressing Gli1 or Gli2 (IC50 = 6 & 4 µM, respectively). HPI-1 is also shown to inhibit the ligand-independent Hh pathway activation in SmoM2-expressing primary murine CGNPs (Cerebellar Granule Neuron Precursors) and NIH 3T3 (smoM2-LIGHT) cultures.

Other Notes

Hyman, J.M., et al. 2009. Proc. Natl. Acad. Sci. USA106, 14132.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

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