373276
Hh Signaling Antagonist XIII, HPI-3
The Hh Signaling Antagonist XIII, HPI-3, also referenced under CAS 796887-98-4, controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.
Synonym(s):
Hh Signaling Antagonist XIII, HPI-3, 2-((-(4-Phenylpiperazin-4-yl)quinazolin-4-yl)amino)ethanol
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About This Item
Empirical Formula (Hill Notation):
C20H23N5O
CAS Number:
Molecular Weight:
349.43
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
solid
Storage condition:
OK to freeze, protect from light
Quality Segment
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
beige
solubility
DMSO: 50 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
A cell-permeable piperazinyl-quinazoline compound that acts as a downstream Hh (Hedgehog) pathway blocker, effectively inhibiting Gli-dependent transcription activity in both Sufu+/+ NIH 3T3 (shh-LIGHT2) and Sufu-/- fibroblast cultures (IC50 = 3 & 9 µM, respectively) in a manner similar to two other Hh downstream blockers, HPI-1 (Cat. No. 373275) and GANT-61 (Cat. No. 373401). However, unlike HPI-1, HPI-3′s inhibitory activity against Su(fu)-/- fibroblasts is completely abolished by Gli1 or Gli2 overexpression. While both HPI-1 and HPI-3 are shown to inhibit the ligand-independent Hh pathway activation in SmoM2-expressing NIH 3T3 cultures (IC50 = 2.5 & 1 µM, respectively), only HPI-1, but not HPI-3, is able to effectively suppress the SmoM2-induced Hh pathway activation in primary murine CGNPs (Cerebellar Granule Neuron Precursors) cultures.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Hyman, J.M., et al. 2009. Proc. Natl. Acad. Sci. USA106, 14132.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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