386041
Anti-Hsp90 Rat mAb (16F1)
liquid, clone 16F1, Calbiochem®
Synonym(s):
Anti-Hsp82, Anti-Hsp85, Anti-Hsp89
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
16F1, monoclonal
Species reactivity:
-
Application:
—
Citations:
-
biological source
rat
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
16F1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSP90AA1(3320)
General description
Protein G purified rat monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Recognizes the ~90 kDa Hsp90 protein.
This Anti-Hsp90 Rat mAb (16F1) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Electron Microscopy for the detection of Hsp90.
Immunogen
Hsp90 isolated from therapeutic human orchiectomy specimens
Human
Application
ELISA (see comments)
Immunoblotting (2 µg/ml, colormetric)
Immunocytochemistry (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Electron Microscopy (see comments)
Immunoblotting (2 µg/ml, colormetric)
Immunocytochemistry (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Electron Microscopy (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Heat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells
Heat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells
Other Notes
Schuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
Lanks, K.W., et al. 1982. J. Biol. Chem.257, 8604.
Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
Lanks, K.W., et al. 1982. J. Biol. Chem.257, 8604.
This antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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