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Merck

391334

BES

ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

Synonym(s):

BES, Free Acid, ULTROL® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

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About This Item

Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
10191-18-1
Molecular Weight:
213.25
UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
MFCD00007533

Key Documents

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SMILES string

[S](=O)(=O)([O-])CC[N+H](CCO)CCO

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

assay

≥99% dry basis (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

pKa (20 °C)

7.12

solubility

water: 1 mg/mL

cation traces

heavy metals: ≤1 ppm

shipped in

ambient

storage temp.

15-25°C

Quality Level

100

Related Categories

Disclaimer

Toxicity: Standard Handling (A)

General description

Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.

Other Notes

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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