401607
Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate
liquid, Calbiochem®
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse
Application:
—
Citations:
2
biological source
goat
Quality Segment
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Enzyme Immunoassay (1:1000)
Immunoelectrophoresis )
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Immunoaffinity purified goat polyclonal antibody conjugated to β-galactosidase. Recognizes mouse IgG, heavy and light chains.
This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain.
Application
Enzyme Immunoassay (1:1000)
Immunoelectrophoresis (see comments)
Immunoelectrophoresis (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM PBS, 10 mM glycine, 10 mM MgCl₂, 1% BSA, pH 7.0.
Other Notes
To achieve maximum enzyme activity, we recommend using the following substrate:
Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl2) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration.
Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.
Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl2) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration.
Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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