407245
Insulin-Like Growth Factor-II, Human, Recombinant, E. coli
Synonym(s):
Insulin-Like Growth Factor-II, Human, Recombinant, E. coli, rhIGF-II
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About This Item
CAS Number:
UNSPSC Code:
12352202
assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1 EU/μg Endotoxin (EU/μg IGF-II)
shipped in
ambient
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
ED₅₀ = 5-10 ng/ml
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human insulin-like growth factor-II expressed in E. coli. A growth-promoting peptide that is structurally related to insulin. IGF-II shares about 76% sequence identity with IGF-I and ~50% with pro-insulin. Its actions are similar to IGF-I (Cat. No. 407240). IGF-II is a nonglycosylated, single chain peptide of 67 amino acids. Plays an important role in fetal development. Stimulates muscle and bone cell proliferation and differentiation.
Other Notes
Feldser, D., et al. 1999. Cancer Res.59, 3915.
Hill, P.A., et al. 1995. Endocrinology136, 124.
Nielson, F.C., et al. 1995. Nature 377, 358.
LeRoith, D., and Roberts, C.R., Jr. 1993. Ann. N.Y. Acad. Sci.692, 1.
Lowe, W.L. 1991. In Insulin-Like Growth Factors: Molecular and Cellular Aspects (LeRoith, D., Ed.) CRC Press Inc., Boca Raton p. 49.
Rotwein, P. 1991. Growth Factors5, 3.
Hill, P.A., et al. 1995. Endocrinology136, 124.
Nielson, F.C., et al. 1995. Nature 377, 358.
LeRoith, D., and Roberts, C.R., Jr. 1993. Ann. N.Y. Acad. Sci.692, 1.
Lowe, W.L. 1991. In Insulin-Like Growth Factors: Molecular and Cellular Aspects (LeRoith, D., Ed.) CRC Press Inc., Boca Raton p. 49.
Rotwein, P. 1991. Growth Factors5, 3.
Physical form
Lyophilized from sterile-filtered acetic acid.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA. Avoid using buffers near the isoelectric point (6.5).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 1
Certificates of Analysis (COA)
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