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407253

Sigma-Aldrich

Insulin-Like Growth Factor-I, Rat, Recombinant, E. coli

Synonym(s):

rIGF-I

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About This Item

UNSPSC Code:
12352202

Assay

≥95% (SDS-PAGE and HPLC)

Quality Level

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.1 ng/μg Endotoxins (ng/μg IGF-I)

shipped in

wet ice

storage temp.

−20°C

General description

Full-length, recombinant, rat insulin-like growth factor I (IGFI) expressed in E. coli. The IGF-I growth factor regulates proliferation and survival signals through the AKT pathway. This growth hormone is useful in the study of cell proliferation and survival signals.

Biochem/physiol Actions

ED₅₀: <10 ng/ml as determined using a type I IGF receptor binding assay; <30 ng/ml as determined by stimulation of protein synthesis in rat L6 myeloblasts.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Reconstitute with H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffers before use.

Other Notes

Clark, R.G., et al. 1997. Endocrinology138, 4316.
Tamura, K., et al. 1989. J Biol Chem. 264, 5616.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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