407253
Insulin-Like Growth Factor-I, Rat, Recombinant, E. coli
Synonym(s):
rIGF-I
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About This Item
UNSPSC Code:
12352202
Assay
≥95% (SDS-PAGE and HPLC)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.1 ng/μg Endotoxins (ng/μg IGF-I)
shipped in
wet ice
storage temp.
−20°C
General description
Full-length, recombinant, rat insulin-like growth factor I (IGFI) expressed in E. coli. The IGF-I growth factor regulates proliferation and survival signals through the AKT pathway. This growth hormone is useful in the study of cell proliferation and survival signals.
Biochem/physiol Actions
ED₅₀: <10 ng/ml as determined using a type I IGF receptor binding assay; <30 ng/ml as determined by stimulation of protein synthesis in rat L6 myeloblasts.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Reconstitute with H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffers before use.
Other Notes
Clark, R.G., et al. 1997. Endocrinology138, 4316.
Tamura, K., et al. 1989. J Biol Chem. 264, 5616.
Tamura, K., et al. 1989. J Biol Chem. 264, 5616.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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K Tamura et al.
The Journal of biological chemistry, 264(10), 5616-5621 (1989-04-05)
Rat insulin-like growth factor-I (IGF-I), a serum polypeptide with growth promoting activity, was isolated from rat serum by a combination of acid/ethanol extraction, affinity chromatography, and a series of reversed phase high performance liquid chromatography, cation exchange, and reversed phase.
R G Clark et al.
Endocrinology, 138(10), 4316-4323 (1997-10-10)
Besides stimulating GH release, some GH secretagogues also release ACTH and adrenal steroids. Several novel classes of potent GH secretagogues have recently been described, and we have now tested their ability to release corticosterone in conscious normal rats. All analogs
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