407254
IGFBP-3, CF, Mouse, Recombinant
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
solid
Assay:
≥95% (SDS-PAGE)
Quality Level
assay
≥95% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
solubility
soluble (Reconstitute to 100 µg/ml in sterile PBS.)
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, mouse insulin-like growth factor binding protein 3 (IGFBP-3) consisting of amino acids 22-291 and expressed in NS0-derived murine myeloma cells with four substitutions: Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro. Mouse IGFBP-3 cDNA encodes a 291 amino acid residue precursor protein with a putative 27 aa residue signal peptide.
Biochem/physiol Actions
ED₅₀ = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.
Physical form
Lyophilized from 0.2 µm filtered Acetonitrile, TFA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS.
Other Notes
Schuller, A.G.P., et al. 1994. Mol. Cell. Endoc.104, 57.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 2
Certificates of Analysis (COA)
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