Interleukin-6, Mouse, Recombinant, E. coli

Name: Interleukin-6, Mouse, Recombinant, <i>E. coli</i>
Brand: MM

Synonym(s):

rmIL-6

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352202

assay

≥97% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

impurities

≤1 EU/μg Endotoxin (EU/μg IL-6)

shipped in

ambient

storage temp.

−70°C

Quality Level

100

Preparation Note

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.

Biochem/physiol Actions

ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line

Disclaimer

Toxicity: Irritant (B)

General description

Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells. Does not inhibit any significant activity on human cells.

Other Notes

Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.

Physical form

Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Establishment of the ELISA for murine soluble gp130, a signal transducer for the IL-6 family cytokine, and its detection in the ascitic fluids of tumor-bearing mice.

T Matsuda et al.

Biochemical and biophysical research communications, 202(1), 637-642 (1994-07-15)

Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein gp130, which associates with the IL-6 receptor alpha chain (IL-6R alpha) in the presence of IL-6. We prepared monoclonal antibodies (mAbs) specific to murine gp130 by immunizing rats and hamsters with

Generation of retroviruses for the overexpression of cytosolic and mitochondrial glutathione reductase in macrophages in vivo.

Marta Kisgati et al.

Cytotechnology, 54(1), 5-14 (2008-11-13)

Retroviral gene transfer and bone marrow transplantation has been used by many investigators to study the role of macrophage proteins in different mouse models of human disease. While this approach is faster and less expensive than generating transgenic mice with