407700
Interleukin-10, Human, Recombinant, S. frugiperda
Synonym(s):
rhIL-1αrhIL-1βrhIL-10
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
assay
≥95% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<100 pg/μg Endotoxin (pg/μg IL-10)
shipped in
ambient
storage temp.
−70°C
Quality Level
Biochem/physiol Actions
ED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines.
Other Notes
Bachis, A., et al. 2001. J. Neurosci.21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol.98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.
Fortunato, S.J., et al. 2001. Obstet. Gynecol.98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.
Physical form
Carrier-free.
Preparation Note
Following reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Reconstitute in sterile water or buffer to a final concentration of 0.1-1 mg/ml as stock solution. Further dilute in buffer such as PBS, 1% BSA.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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