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420092

Sigma-Aldrich

PhosphoDetect Anti-JAK1 (pTyr1022/1023) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human

should not react with

rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

phosphorylation (pTyr1022/pTyr1023)

Gene Information

human ... JAK1(3716)

General description

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK1 protein phosphorylated on Tyr1022/1023.
Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.
This PhosphoDetect Anti-JAK1 (pTyr1022/1023) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK1 (pTyr1022/1023).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)

Application

Immunoblotting (1:1000)

Physical form

In Dulbecco′s PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.

Preparation Note

Following intitial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
3T3L1 cells treated with LIF

Other Notes

A ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
Liu, K.D., et al. 1997. Curr. Biol.7, 817.
Gauzzi, M.C., et al. 1996. J. Biol. Chem.271, 20494.

Legal Information

Manufactured by Biosource International.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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