420108
JNK Substrate, c-Jun 1-79
Synonym(s):
SAPK Substrate
form
liquid
assay
≥90% (SDS-PAGE)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
Quality Level
Biochem/physiol Actions
Cell permeable: no
Primary Target
Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK)
Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK)
Product does not compete with ATP.
Reversible: no
Shown to undergo phosphorylation by JNK in the presence of ³²P-ATP.
Disclaimer
Toxicity: Standard Handling (A)
General description
GST fusion protein of c-jun (1-79) activation domain, expressed in E. coli. Highly specific substrate form c-jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Not phosphorylated by MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity.
Recombinant c-Jun (1-79) activation domain fused to GST and expressed in E. coli. Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Is not phosphorylated by p42 MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity. Shown to undergo phosphorylation by JNK in the presence of 32P-ATP.
Other Notes
Derijard, B., et al. 1994. Cell 76, 1025.
Kyriakis, J.M., et al. 1994. Nature369, 156.
Hibi, M., et al. 1993. Genes Dev.7, 2135.
Kyriakis, J.M., et al. 1994. Nature369, 156.
Hibi, M., et al. 1993. Genes Dev.7, 2135.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 2 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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