420127
Jasplakinolide
from Jaspis johnstoni, ≥90% (HPLC), liquid, Actin polymerization promotor, Calbiochem
Synonym(s):
InSolution Jasplakinolide, Jaspis johnstoni
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Empirical Formula (Hill Notation):
C36H45BrN4O6
Molecular Weight:
709.67
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥90% (HPLC)
Form:
liquid
Quality level:
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Product Name
Jasplakinolide, Jaspis johnstoni, InSolution, ≥90%, 1 mM in DMSO, mammablian cell mitosis inhibitor, potent inducer of actin polymerization and stabilization
Quality Level
assay
≥90% (HPLC)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
shipped in
wet ice
storage temp.
−20°C
General description
A cell-permeable F-actin probe. This macrocyclic cyclodepsipeptide, isolated from the marine sponge Jaspis johnstoni, is a potent inducer of actin polymerization and stabilization in vitro. It competes with Phalloidin (Cat. No. 516640) for actin binding (Kd = 15 nM) and enhances apoptosis induced by interleukin 2 (IL-2) deprivation. Also displays fungicidal, insecticidal, and anticancer properties.
Biochem/physiol Actions
Cell permeable: no
Kd = 15 nM for actin binding
Primary Target
F-actin
F-actin
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
A 1 mM (50 µg/71 µl) solution of Jasplakinolide, Jaspis johnstoni (Cat. No. 420107) in DMSO.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Rosado, J.A., et al. 2000. J. Biol. Chem.275, 7527.
Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.
Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.
Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.
Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.
Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.
Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.
Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.
Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F - closed cup - (Dimethylsulfoxide)
flash_point_c
87 °C - closed cup - (Dimethylsulfoxide)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Anna M Koester et al.
Biomolecules, 12(8) (2022-07-28)
Recent work suggests that Ras small GTPases interact with the anionic lipid phosphatidylserine (PS) in an isoform-specific manner, with direct implications for their biological functions. Studies on PS-Ras associations in cells, however, have relied on immuno-EM imaging of membrane sheets.
Kem A Sochacki et al.
Developmental cell, 56(8), 1131-1146 (2021-04-07)
Clathrin-mediated endocytosis is the primary pathway for receptor and cargo internalization in eukaryotic cells. It is characterized by a polyhedral clathrin lattice that coats budding membranes. The mechanism and control of lattice assembly, curvature, and vesicle formation at the plasma
Kelli L Hvorecny et al.
Nature communications, 15(1), 1840-1840 (2024-02-29)
The cytoskeletal protein actin plays a critical role in the pathogenicity of the intracellular parasite, Toxoplasma gondii, mediating invasion and egress, cargo transport, and organelle inheritance. Advances in live cell imaging have revealed extensive filamentous actin networks in the Apicomplexan