Skip to Content
Merck
All Photos(1)

Key Documents

434629

Sigma-Aldrich

Anti-Lewis b Mouse mAb (T218)

liquid, clone T218, Calbiochem®

Synonym(s):

Anti-Le b, Anti-Leb

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

T218, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

General description

Mouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
Recognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
This Anti-Lewis b Mouse mAb (T218) is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Lewis b.

Immunogen

Human
human SK-CO-10 colon cancer cells

Application

ELISA (see comments)

Immunoblotting (see comments)

Immunohistochemistry (1:10-1:40)

Physical form

In PBS, 1% BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Colon adenocarcinoma tissue

Other Notes

Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
For immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service