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Merck

434629

Anti-Lewis b Mouse mAb (T218)

liquid, clone T218, Calbiochem®

Synonym(s):

Anti-Le b, Anti-Leb

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About This Item

UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Lewis b Mouse mAb (T218), liquid, clone T218, Calbiochem®

biological source

mouse

antibody product type

primary antibodies

clone

T218, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Analysis Note

Positive Control
Colon adenocarcinoma tissue

Application

ELISA (see comments)

Immunoblotting (see comments)

Immunohistochemistry (1:10-1:40)

Disclaimer

Toxicity: Standard Handling (A)

General description

Mouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
Recognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
This Anti-Lewis b Mouse mAb (T218) is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Lewis b.

Immunogen

Human
human SK-CO-10 colon cancer cells

Other Notes

Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
For immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.

Physical form

In PBS, 1% BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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