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434636

Sigma-Aldrich

Anti-Lewis y Mouse mAb (F3)

liquid, clone F3, Calbiochem®

Synonym(s):

Anti-Le y, Anti-Ley

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

F3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FUT3(2525)

General description

Purified mouse monoclonal antibody. Recognizes the Lewis y blood group antigen.
Recognizes the Lewis y carbohydrate chain in colon adenocarcinoma.
This Anti-Lewis y Mouse mAb (F3) is validated for use in Immunohistochemistry for the detection of Lewis y.

Immunogen

Human
human SK-LU-3 lung cancer cells

Application

Immunohistochemistry (1:10-1:120)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1% BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Colon adenocarcinoma tissue

Other Notes

Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
For immunohistochemistry, fix tissue in 10% formalin buffered to neutral pH. Variables associated with assay conditions will dictate the proper working dilutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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