444232
MMP-9, Dimer, Human Neutrophil
Synonym(s):
Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
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About This Item
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥1000 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
foreign activity
other MMP activity, none detected
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.
M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.
Other Notes
Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
Physical form
In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% 35 Detergent, 0.05% NaN₃, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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