454849
MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli
Synonym(s):
ERK1, MAPK3, p44 MAPK
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.54
recombinant
expressed in E. coli
Quality Level
Assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥6000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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