454854
MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli
Synonym(s):
ERK2, MAPK2, p42 MAPK, ERK2, MAPK2, p42MAPK
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About This Item
UNSPSC Code:
12352202
Specific activity:
≥5500 units/mg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥5500 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Physical form
In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Lew, J. 2003. Biochemistry42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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