475901
Monocyte Chemotactic Protein-1, Human, Recombinant, E. coli
A potent chemoattractant for human monocytes and mouse peritoneal macrophages. Its effects are proposed to be mediated by P2-integrins.
Synonym(s):
rhMCP-1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥97% (SDS-PAGE)
Quality Segment
assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotoxins (EU/μg MCP-1)
shipped in
ambient
storage temp.
−70°C
General description
Recombinant, human monocyte chemotactic protein-1 expressed in E. coli. A potent chemoattractant for human monocytes and mouse peritoneal macrophages. Its effects are proposed to be mediated by P2-integrins.
Biochem/physiol Actions
ED₅₀ = 5-20 ng/ml as measured by its monocyte chemotactic activity
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg MCP-1.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
Other Notes
Hosang, K., et al. 1994. Biochem. Biophys. Res. Commun.199, 962.
Luini, W., et al. 1994. Cytokine 6, 28.
Luini, W., et al. 1994. Cytokine 6, 28.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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