475938
MT2-MMP, Catalytic Domain, Human, Recombinant, E. coli
Synonym(s):
MMP-15 Catalytic Domain
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥30 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Note: 1 mU = 1 milliunit.
Recombinant, human pro-MT2-MMP expressed in E. coli as a periplasmic protein and activated to yield the active catalytic domain (amino acids 91-267). The catalytic domain is comprised of amino acid residues Tyr91 to Gly267 of mature human MT2-MMP. MT2-MMP is used to study the activation of MMP-2 and the degradation of extracellular matrix proteins.
Other Notes
D’Ortho, M.-P., et al. 1997. Eur. J. Biochem.250, 751.
Kolkenbrock, H., et al. 1997. Biol. Chem.378, 71.
Knight, C.G., et al. 1992. FEBS Lett. 296, 263.
Kolkenbrock, H., et al. 1997. Biol. Chem.378, 71.
Knight, C.G., et al. 1992. FEBS Lett. 296, 263.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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