480275
β-NGF, Human, Recombinant, NSO Cells
Synonym(s):
β-Nerve Growth Factor, Human, Recombinant
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥97% (SDS-PAGE)
Recombinant:
expressed in NSO cells
recombinant
expressed in NSO cells
Quality Segment
assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤100 pg/μg Endotoxin (pg/μg β-NGF)
shipped in
ambient
storage temp.
−70°C
General description
Recombinant, human β-nerve growth factor expressed in NSO cells. Involved in neuroimmune interactions and inflammation.
Biochem/physiol Actions
ED₅₀ = 800-1500 pg/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line
Physical form
Lyophilized from sterile-filtered solution in PBS containing 50 µg BSA/µg β-NGF.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
Other Notes
Leon, A., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3739.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
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