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Merck

480716

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

Synonym(s):

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, N-Acetylneuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:
9001-67-6
EC Number:
3.2.1.18 (BRENDA, IUBMB)
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
MFCD00040440

Key Documents

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recombinant

expressed in E. coli

description

RTECS - QQ3450000

form

liquid

specific activity

≥40 units/mg protein
≥5 units/mL

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

Related Categories

Disclaimer

Toxicity: Harmful (C)

General description

Note: 1 mU = 1 milliunit.
Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).

Physical form

In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Sarah W Nelson et al.
Influenza and other respiratory viruses, 13(6), 593-602 (2019-08-09)
The influenza A virus (IAV) binds to α-2,3- and α-2,6-linked sialic acid (SA) receptors expressed by Madin-Darby canine kidney (MDCK) cells. The receptor distribution may therefore be important in regulating IAV propagation. Serum-free medium (SFM) avoids variability in conventional culture
Karima S Khimani et al.
Scientific reports, 10(1), 2933-2933 (2020-02-23)
To compare goblet cell (GC) number and area in the covered superior (SB) versus exposed temporal (TB) bulbar conjunctiva in control versus aqueous tear deficient eyes (ATD) and evaluate correlation with tear MUC5AC protein. SB and TB impression cytology performed

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