481974
NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda
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assay
≥90% (SDS-PAGE)
Quality Segment
form
liquid
specific activity
≥20 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
solubility
aqueous buffer: soluble
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratio 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane-bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratios ranging from 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
One unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome c per min at 20°C, pH 7.7.
Shen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
Yamano, S., et al. 1989. Mol. Pharmacol.36, 83.
Yamano, S., et al. 1989. Mol. Pharmacol.36, 83.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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