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Merck

487655

p-Nitrophenyl Phosphate, DiTris Salt

Excellent substrate for alkaline phosphatase-based ELISA assays.

Synonym(s):

p-Nitrophenyl Phosphate, DiTris Salt, pNPP, 2Tris

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About This Item

Empirical Formula (Hill Notation):
C14H28N3O12P
CAS Number:
Molecular Weight:
461.36
UNSPSC Code:
41116158
NACRES:
NA.77
MDL number:
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Product Name

p-Nitrophenyl Phosphate, DiTris Salt, Excellent substrate for alkaline phosphatase-based ELISA assays.

SMILES string

[P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-].[N+H3]C(CO)(CO)CO.[N+H3]C(CO)(CO)CO

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.05% Free-p-Nitrophenol

color

off-white to pale yellow

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

Quality Level

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for alkaline phosphatase-based ELISA assays
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.

Preparation Note

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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