5.00499
PI 3-K Inhibitor XVIII
Synonym(s):
PI 3-K Inhibitor XVIII, Myr-N-KAAEIASSDSANVSSRGGKKFLPV-NH2, p110β-Gβγ Interaction Inhibitor peptide, Cell-permeable
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About This Item
Empirical Formula (Hill Notation):
C118H202N32O35
Molecular Weight:
2629.06
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
solid
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Quality Segment
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
white
solubility
H2O: 25 mg/mL
storage temp.
2-8°C
General description
A PI 3-K p110β-derived Gβγ-binding peptide (aa 514-537 in p110β C2-helical linker region) is made cell-permeable via N-myristoylation and prevents Gβγ-, but not RTK-, mediated p110β activation both in cell-free immune complex activation assays (complete inhibition by 1 µM peptide) and in cultures (complete inhibition of pAKT T308 by 30 min 30 µM peptide treatment of p110β/p85/Akt/Gβγ-transfected HEK 293E cells) without affecting basal p110β activity, p110β-Rab5 interaction, nor Gαβ-dependent activation of adenylyl cyclase or p101-p110γ dimer. The ATP-competitive p110β inhibitior TGX-221 (Cat. No. 528113) can be used in conjunction for studying Gβγ-dependency of p110β-mediated cellular functions.
An N-myristoylated cell-permeable PI 3-K p110β-derived Gβγ-binding peptide (aa 514-537 in p110β C2-helical linker region) that prevents Gβγ-, but not RTK-, mediated p100β activation both in cell-free assays (1 µM) and in cultures (30 µM in p110β/p85/Akt/Gβγ-transfected HEK 293E cells) without affecting basal p100β activity, p110β-Rab5 interaction, nor Gβγ-dependent activation of adenylyl cyclase or p101-p110γ dimer. The ATP-competitive p110β inhibitior TGX-221 (Cat. No. 528113) can be used in conjunction for studying Gβγ dependency of p110β-mediated cellular functions.
Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Gβγ subunit of G-proteins
Gβγ subunit of G-proteins
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Dbouk, H.A., et al. 2012. Sci. Signal. 5, ra89.
N-Myr-Lys-Ala-Ala-Glu-Ile-Ala-Ser-Ser-Asp-Ser-Ala-Asn-Val-Ser-Ser-Arg-Gly-Gly-Lys-Lys-Phe-Leu-Pro-Val-NH₂
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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