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Merck

5.05224

Necrostatin-1

InSolution, ≥95%, 25 mM in DMSO, required for the biosynthesis of sialic acid-containing complex carbohydrates

Synonym(s):

InSolution Necrostatin-1, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protei, Necrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I

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About This Item

Empirical Formula (Hill Notation):
C13H13N3OS
Molecular Weight:
259.33
NACRES:
NA.77
UNSPSC Code:
12352200

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assay

≥99% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light

storage temp.

−70°C

Quality Level

100

Related Categories

General description

A cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect on RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (>Cat. No. 480066).

Biochem/physiol Actions

Cell permeable: yes
Reversible: yes

Packaging

Packaged under inert gas

Physical form

A 25 mM (10 mg/1.54 ml) sterile-filtered solution of Necrostatin-1 (Cat. No. 480065) in DMSO.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Other Notes

Degterev, A., et al. 2013. Nat. Chem. Biol.9, 192.
Degterev, A., et al. 2012. Cell Death Differ.20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis.3, e320.
Takahashi, N., et al. 2012. Cell Death Dis.3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol.4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol.1, 112.
Muller, A.J., et al. 2005. Nat. Med.11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett.15, 5039.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - (refers to pure substance)

flash_point_c

87 °C - (refers to pure substance)

Certificates of Analysis (COA)

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