5.06075
Novel Inducer of Death-1, NID-1
Synonym(s):
Novel Inducer of Death-1, NID-1, (E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
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About This Item
Empirical Formula (Hill Notation):
C15H14N2O3S
CAS Number:
Molecular Weight:
302.35
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
solid
Quality level:
Storage condition:
OK to freeze, protect from light
assay
≥98% (HPLC)
Quality Level
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
yellow
solubility
DMSO: 50 mg/mL
storage temp.
2-8°C
General description
A cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC50 = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington’s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Varma, H., et al. 2013. Exp. Cell Res.319, 1759.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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