5.06075
Novel Inducer of Death-1, NID-1
Synonym(s):
Novel Inducer of Death-1, NID-1, (E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
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About This Item
Empirical Formula (Hill Notation):
C15H14N2O3S
CAS Number:
Molecular Weight:
302.35
UNSPSC Code:
12352200
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
yellow
solubility
DMSO: 50 mg/mL
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
A cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC50 = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington’s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.
Other Notes
Varma, H., et al. 2013. Exp. Cell Res.319, 1759.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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