Assay
≥97% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, mouse placenta growth factor 2 (PlGF-2), consisting of amino acids 24-158 and 27-158, and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. PlGF-2 is a member of the VEGF family of growth factors.
Biochem/physiol Actions
Receptor binding: 0.1-5 ng/ml as measured in a functional ELISA with 2 µg/ml (100 µg/well) immobilized rmFlt-1/Fc chimera.
Physical form
Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute 50 100 µg/ml with sterile PBS contanining ≥1% human or bovine serum albumin.
Other Notes
Cao, Y. et al. 1997. Biochem. Biophys. Res. Commun.235, 493.
Ferrara, N. et al. 1997. Endocrin. Rev.18, 4.
DiPalma, T. et al. 1996. Mamm. Genome7, 6.
Ferrara, N. et al. 1997. Endocrin. Rev.18, 4.
DiPalma, T. et al. 1996. Mamm. Genome7, 6.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
4.1B - Flammable solid hazardous materials
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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