PDGF, AA Homodimer, Human, Recombinant, E. coli

Toxicity: Standard Handling (A)

1-3 ng/ml

Recombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that deduced from the native human nucleotide sequence except for the addition of an N-terminal methionine group.

Butt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Betsholtz, C., et al. 1986. Nature320, 695.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.

Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.

Reconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.

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