521270
PDK1, His•Tag®, Human, Recombinant, S. frugiperda
Synonym(s):
3-Phosphoinositide-Dependent Protein Kinase-1
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About This Item
UNSPSC Code:
12352202
EC Number:
2.7.10
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥75 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Full length, recombinant, human PDK1 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
Other Notes
One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
Scott, M.T., et al. 2002. EMBO J.21, 6771.
Biondi, R.M., et al. 2000. EMBO J.19, 979.
Alessi, D.R., et al. 1997. Curr. Biol.7, 776.
Biondi, R.M., et al. 2000. EMBO J.19, 979.
Alessi, D.R., et al. 1997. Curr. Biol.7, 776.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
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