524545
Phosphatase, Alkaline, E. coli
Synonym(s):
Phosphatase, Alkaline, E. coli
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About This Item
Specific activity:
≥500 units/mL
form
liquid
Quality Level
specific activity
≥500 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
pI
4.5
foreign activity
Non-specific endonuclease, RNase, none detected
shipped in
wet ice
storage temp.
−20°C
General description
Native alkaline phosphatase from E. coli. A dimeric zinc- and magnesium-containing protein. A non-specific phosphomonoesterase with a pI of 7.5.
Physical form
In 50 mM Tris-HCl, 50% glycerol, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of pNPP per min at 25°C in Tris-HCl, pH 8.0.
Reid, T.W. and Willson, I.B. 1971. Enzymes4, 383.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
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