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Merck

524604

Phosphatase, Alkaline, Human Placenta

Synonym(s):

Phosphatase, Alkaline, Human Placenta

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:

form

liquid

specific activity

≥100 U/mg
≥300 units/mL

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

Quality Level

Disclaimer

Toxicity: Standard Handling (A)

General description

Native alkaline phosphatase from human placenta. Homodimeric enzyme that hydrolyzes phosphate esters of primary and secondary alcohols, amines, and phenols. Useful in the hydrolysis of 5′-terminal phosphates of DNA and RNA. Active site contains a reactive serine.

Other Notes

Fishman, W.H., et al. 1973. Anal. Biochem.51, 368.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 μmol pNPP per min at 37°C, pH 10.15.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 50% glycerol, pH 7.6.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1


Certificates of Analysis (COA)

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