524734
Phosphodiesterase 3B, His•Tag®, Human, Recombinant, S. frugiperda
Synonym(s):
PDE3B, cGMP-inhibited phosphodiesterase 3B
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
specific activity
≥0.5 U/μL
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Full-length, recombinant, human phosphodiesterase 3B (accession no. AY459346) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. An important component of insulin and cAMP-dependent signaling. Supplied as a partially purified preparation.
Other Notes
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.
One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.
Physical form
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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