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Key Documents

525320

Sigma-Aldrich

PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

liquid, clone PY20, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

PY20, monoclonal

form

liquid

contains

0.1% Proclin as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(ELISA (1:2000)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500))

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

phosphorylation (pTyr)

Gene Information

human ... PID1(55022)

General description

Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Application

ELISA (1:2000)

Immunoblotting (1:500-1:2000)

Immunohistochemistry (1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol, 1% BSA, pH 7.4.

Other Notes

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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