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Merck

529659

DDD00033325

≥95% (HPLC), syrup, single hESC survival promoter, Calbiochem®

Synonym(s):

Pro-survival Compound, DDD00033325, DDD00033325

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About This Item

Empirical Formula (Hill Notation):
C17H19N5
CAS Number:
1259034-82-6
Molecular Weight:
293.37
UNSPSC Code:
51111800
NACRES:
NA.77

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Product Name

Pro-survival Compound, DDD00033325, A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.

InChI

1S/C17H19N5/c1-2-4-14(5-3-1)12-21-10-7-15(13-21)19-16-8-11-22-17(20-16)6-9-18-22/h1-6,8-9,11,15H,7,10,12-13H2,(H,19,20)/t15-/m1/s1

InChI key

ZJVYTXWKNDTQNC-OAHLLOKOSA-N

SMILES string

C12=CC=NN1C=CC(N[C@@H]3CCN(CC4=CC=CC=C4)C3)=N2

assay

≥95% (HPLC)

form

syrup

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 100 mg/mL

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Related Categories

Disclaimer

Toxicity: Regulatory Review (Z)

General description

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC50 = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

Other Notes

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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