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Merck

539154

20S Proteasome, Rabbit

20S Proteasome is a catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Fully functional with Z-LLVY-AMC as the substrate.

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About This Item

UNSPSC Code:
12352202

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Product Name

20S Proteasome, Rabbit, 20S Proteasome is a catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Fully functional with Z-LLVY-AMC as the substrate.

assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Biochem/physiol Actions

Fully functional as measured using Z-LLVY-AMC as the substrate following SDS activation

Disclaimer

Toxicity: Standard Handling (A)

General description

Native, rabbit 20S proteasome. Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Can be activated by PA28 (Cat. No. 506280) or SDS.

Other Notes

Stein, R.L., et al. 1996. Biochemistry 35, 3899.
Ciechanover, A. 1994. Cell 79, 13.
Dick, L.R., et al. 1994. J. Immunol. 152, 3884.
Dick, L.R., et al. 1991. Biochemistry 30, 2725.
Hough, R., et al. 1987. J. Biol. Chem. 262, 8303.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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