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539166

Sigma-Aldrich

Anti-19S Proteasome S2-Subunit Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Recognizes the ~100 kDa 19S proteasome S2-subunit in HeLa cells and partially purified 26S proteasome preparations. Also recognizes an ~50 kDa potential subunit degradation product.

Immunogen

Human
recombinant, human 19S proteasome S2-subunit

Application

Immunoblotting (1:1000)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells

Other Notes

Also detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Kloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol.2, 179.
Yew, P.R. 2001. J. Cell Physiol.187, 1.
Chaturvedi, K., et al. 2000. Biopolymers55, 334.
Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys.383, 1.
Pickart, C.M. 2000. Trends Biochem Sci.25, 544.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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