539305
Protein G, Alkaline Phosphatase Conjugate
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About This Item
NACRES:
NA.41
UNSPSC Code:
41116133
Form:
liquid
Biological source:
(Streptococcus species)
biological source
(Streptococcus species)
Quality Level
form
liquid
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Direct Immunoassay (1:2500))
shipped in
wet ice
storage temp.
2-8°C
General description
Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.
Protein G, Alkaline Phosphatase Conjugate is obtained by conjugation of protein G, a cell-surface protein of Streptococcus species to alkaline phosphatase. This conjugate is used as a secondary agent in immunoassay methods to detect polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a, and IgG2b.
Application
Protein G, Alkaline Phosphatase Conjugate has been used in:
- direct enzyme-linked immunosorbent assay (ELISA) (1:1000)(1:250)
- indirect ELISA (1:1000)
- immunoblot (1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
Analysis Note
Single band at 33.8 kDa by SDS-PAGE
Other Notes
p-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S Sun et al.
Journal of immunological methods, 152(1), 43-48 (1992-07-31)
The IgG binding domains of staphylococcal protein A and streptococcal protein G were expressed as a chimaera using the pGEX vector which has been advocated because its fusion proteins tend to be soluble and easily isolated on immobilised glutathione. This
A O Olarinmoye et al.
Epidemiology and infection, 145(10), 2030-2037 (2017-05-10)
The hamadryas baboon (Papio hamadryas hamadryas) is the only indigenous species of non-human primates (NHP) found in the Kingdom of Saudi Arabia (KSA). There are no peer-reviewed publications on viral infections of the baboons of KSA. Apart from camels, other
B Nilson et al.
Journal of immunoassay, 9(2), 207-225 (1988-01-01)
Protein G, an IgG-binding protein, purified from the surface of group G streptococci, was coupled to alkaline phosphatase. The conjugate was used for detection of polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a and IgG2b in an enzyme-linked