539568
PP2B, Human, Recombinant, E. coli
Synonym(s):
CaN, Protein Phosphatase 2B, Calcineurin
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
form
liquid
specific activity
≥300,000 U/mg
≥50,000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
Analysis Note
Two distinct bands by SDS-PAGE
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
Other Notes
Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry36, 7418.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry36, 7418.
One unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.
Physical form
In 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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